منابع مشابه
Increased mir33 Expression in Expanded Hematopoietic Stem Cells Cultured on Adipose Stem Cells Feeder layer
Bachgroun: Hematopoietic stem cell derived from umbilical cord blood (UCB) has been used for regenerative medicine in hematological abnormalities. MicroRNAs are important regulators of gene expression that control both physiological and pathological processes such as development of tissue and cancer. Some studies have shown that miR-33, has a critical role in control of self-renewal cells. He...
متن کاملDifferentiation of Mouse Embryonic Stem Cells into Hematopoietic Cells
Purpose: Differentiation of Mouse embryonicstem cells into Hematopoietic cells. Materials and Methods: In this study, we used EB formation system for Hematopoietic differentiation of mouse embryonic stem cell (Royan B1) in suspension culture. EBs cultured in medium with Hematopoietic inducer cytokines (SCF, TPO, GMCSF, IL3, Flt3 and EPO) .presence of hematopoietic differentiated cell assessed ...
متن کاملincreased mir33 expression in expanded hematopoietic stem cells cultured on adipose stem cells feeder layer
bachgroun: hematopoietic stem cell derived from umbilical cord blood (ucb) has been used for regenerative medicine in hematological abnormalities. micrornas are important regulators of gene expression that control both physiological and pathological processes such as development of tissue and cancer. some studies have shown that mir-33, has a critical role in control of self-renewal cells. here...
متن کاملEngraftment of cultured human hematopoietic cells in sheep.
In an effort to expand human hematopoietic progenitors and stem cells in vitro, we cultured human CD34(+)c-kitlow bone marrow cells in suspension in the presence of KIT ligand, FLK2/FLT3 ligand, interleukin-6 (IL-6), and erythropoietin with or without IL-3 and tested their engrafting capabilities by injecting them into sheep fetuses. As markers for engraftment, we analyzed CD45(+) cells and kar...
متن کاملUltrastructural studies relating to the surface morphology of cultured cells.
Scanning electron microscopy and a surface-replica technique for transmission electron microscopy have been used to study the ultrastructural features of cultured-cell surfaces The presence of microvilh measuring 0-1-0-2 fim in diameter by up to 5 ftm in length has been noted as a regular feature of Landschutz ascites, ' fibroblastic' HeLa, and canine kidney, cells. The surfaces of chick mesenc...
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ژورنال
عنوان ژورنال: Cancer
سال: 1968
ISSN: 0008-543X,1097-0142
DOI: 10.1002/1097-0142(196808)22:2<245::aid-cncr2820220202>3.0.co;2-1